A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity

A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity

The present work describes the construction and validation of a human scFv library with a novel design approach to synthetic complementarity determining region (CDR) diversification. The advantage of synthetic antibody libraries includes the possibility of exerting fine control over factors like framework sequences, amino acid and codon usage, and CDR diversity. However, random combinatorial sy...

Limited diversity of human scFv fragments isolated by panning a synthetic phage-display scFv library with cultured human melanoma cells.

To broaden the specificity of the Abs recognizing human melanoma-associated Ags (MAAs), we have isolated human single-chain fragment of the V region (scFv) fragments by panning the synthetic phage Ab library (#1) with the human melanoma cell lines S5 and SK-MEL-28. All of the isolated scFv fragments reacted with the mouse mAb defined high molecular weight melanoma-associated Ag (HMW-MAA). scFv ...

Human Melanoma Cells Phage-Display scFv Library with Cultured Isolated by Panning a Synthetic Limited Diversity of Human scFv Fragments

Discovery of a novel synthetic phosphatase from a bead-bound combinatorial library.

Using split/pool encoded synthesis and a colorimetric catalysis assay, a number of synthetic phosphatase catalysts were developed.

Identification of novel hexapeptides bioactive against phytopathogenic fungi through screening of a synthetic peptide combinatorial library.

The purpose of the present study was to improve the antifungal activity against selected phytopathogenic fungi of the previously identified hexapeptide PAF19. We describe some properties of a set of novel synthetic hexapeptides whose D-amino acid sequences were obtained through screening of a synthetic peptide combinatorial library in a positional scanning format. As a result of the screening, ...